salmonella enterica serovar enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination

نویسندگان

g. z. he

s. x. deng

w. y. tian

چکیده

serovar-specific real-time pcr for salmonella enterica serovar enteritidis (s. enteritidis) was conductedto detect the genomic dna of s. enteritidis from laying goose after subcutaneous vaccination at differenttime points. indirect fluorescent antibody (ifa) technique and immunohistochemical localization wereemployed to validate the results. the results showed that s. enteritidis was consistently detected in all thesamples. vagina and uterus were positive at 20 h pi, and the last organ to show a positive result was thelargest and third largest preovulatory follicle, at 32 h pi. the copy numbers of s. enteritidis dna in eachtissue reached a peak at 36-60 h pi, with the vagina and uterus containing higher concentrations than othertissues. however, the number of bacteria started decreasing by 3-4 d, and by 6 d, the concentration of s.enteritidis dna was below the detection limits of the pcr assay, except the vagina. the real-time pcranalysis of a variety of tissues is significant for further investigation of the mechanism of vaccine protectionand the optimization of vaccination regimes.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination

Serovar-specific real-time PCR for Salmonella enterica serovar Enteritidis (S. Enteritidis) was conductedto detect the genomic DNA of S. Enteritidis from laying goose after subcutaneous vaccination at differenttime points. Indirect fluorescent antibody (IFA) technique and immunohistochemical localization wereemployed to validate the results. The results showed that S. Enteritidis was consistent...

متن کامل

Control of Salmonella enterica serovar Enteritidis in laying hens by inactivated Salmonella Enteritidis vaccines

Salmonella Enteritidis is one of the agents that is responsible for outbreaks of human foodborne salmonellosis caused by Salmonella Enteritidis and is generally associated with the consumption of poultry products. Inactivated Salmonella Enteritidis cell vaccine is one of the available methods to control Salmonella Enteritidis in breeders and laying hens, however results in terms of efficacy var...

متن کامل

Salmonella enterica Serovar Enteritidis, Japan

To the Editor: Nontyphoidal sal-monellae are the important causative agents of foodborne diseases in Japan and other industrialized countries. Salmonella enterica serovar Enter-itidis has risen to the leading cause of infection among Salmonella spp. since 1989 (1). Emergence of drug-resistant S. Enteritidis has been rarely reported while S. Typhimurium, another serovar of major public health co...

متن کامل

Immunoprotectivity of Salmonella enterica serovar Enteritidis virulence protein, InvH, against Salmonella typhi

Objective(s):Typhoid fever is a dreadful disease of a major threat to public health in developing countries. Vaccination with bacterial immunodominant components such as surface proteins may prove as a potent alternative to live attenuated vaccines. InvH, an important part of needle complex in type three secretion system (TTSS) plays important role in efficient bacterial adherence and entry int...

متن کامل

A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals.

Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic DeltaguaB mutant, a nonflagellated DeltafliC mutant, and an auxotrophic and nonflagellated DeltaguaB DeltafliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 14

شماره 2 2013

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023